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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become noticed in many tumors, which results in chromosome instability, and so may well lead to tumorigenesis. This protein was discovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a important purpose in marketing mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, are explained. Two further splice variants were described but in depth nucleotide sequence information will not be however out there.
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Blocking peptides are peptides that bind specially to the goal antibody and block antibody binding. These peptide ordinarily consists of the epitope acknowledged via the antibody.
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This information outlines further more feasible optimization of mobile and immunoassay parameters to ensure the best possible final results are...
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This guide outlines more attainable optimization of cellular and immunoassay parameters to make sure the absolute best success are...
Antibodies certain to the blocking peptide no longer bind into the epitope over the concentrate on protein. By evaluating the staining from your blocked antibody compared to the antibody alone, you can see which staining is particular.
Target Information Cyclin E belongs into the really conserved cyclin household, whose associates are characterised by a spectacular periodicity in protein abundance in the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins exhibit unique expression and degradation patterns which lead to the temporal coordination of each and every mitotic party. Cyclin E kinds a posh with and functions to be a regulatory subunit of CDK2, whose activity is needed for cell cycle G1/S changeover. This protein accumulates in the G1-S stage boundary which is degraded as cells progress by way of S section.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by equally antibodies...
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